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Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols

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ISBN: 9783319162584 9783319162591 Year: Pages: 35 DOI: 10.1007/978-3-319-16259-1 Language: English
Publisher: Springer Nature
Subject: Biotechnology --- Plant Sciences --- Manufactures --- Chemical Engineering --- Pharmacy and materia medica
Added to DOAB on : 2016-04-12 17:07:01
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Open Access provided with a grant from the International Atomic Energy Agency.This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

Ship and Offshore Structure Design in Climate Change Perspective

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Book Series: SpringerBriefs in Climate Studies ISSN: 2213784X ISBN: 9783642341373 9783642341380 Year: Pages: 69 DOI: http://dx.doi.org/10.1007/978-3-642-34138-0 Language: English
Publisher: Springer Nature
Subject: Geology --- Earth Sciences --- Transportation --- Pharmacy and materia medica --- Environmental Sciences --- General and Civil Engineering --- Electrical and Nuclear Engineering --- Materials --- Business and Management --- Biotechnology
Added to DOAB on : 2013-09-04 12:15:49
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This book summarizes results of longstanding research and scientific contributions from many projects and relevant working groups. It collects and evaluates wind and wave climate projections under changing climate having design needs and marine safety in focus. Potential impact of projected climate change in met-ocean conditions on ships and offshore structures is discussed and illustrated by an example of the expected wave climate change on tanker design.

Ocean-Atmosphere Interactions of Gases and Particles

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Book Series: Springer Earth System Sciences ISSN: 21979596 ISBN: 9783642256424 9783642256431 Year: Pages: 315 DOI: 10.1007/978-3-642-25643-1 Language: English
Publisher: Springer Nature
Subject: Business and Management --- General and Civil Engineering --- Materials --- Geography
Added to DOAB on : 2014-02-10 16:18:02
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The oceans and atmosphere interact through various processes, including the transfer of momentum, heat, gases and particles. In this book leading international experts come together to provide a state-of-the-art account of these exchanges and their role in the Earth-system, with particular focus on gases and particles. Chapters in the book cover: i) the ocean-atmosphere exchange of short-lived trace gases; ii) mechanisms and models of interfacial exchange (including transfer velocity parameterisations); iii) ocean-atmosphere exchange of the greenhouse gases carbon dioxide, methane and nitrous oxide; iv) ocean atmosphere exchange of particles and v) current and future data collection and synthesis efforts. The scope of the book extends to the biogeochemical responses to emitted / deposited material and interactions and feedbacks in the wider Earth-system context. This work constitutes a highly detailed synthesis and reference; of interest to higher-level university students (Masters, PhD) and researchers in ocean-atmosphere interactions and related fields (Earth-system science, marine / atmospheric biogeochemistry / climate). Production of this book was supported and funded by the EU COST Action 735 and coordinated by the International SOLAS (Surface Ocean- Lower Atmosphere Study) project office.

Radiation Monitoring and Dose Estimation of the Fukushima Nuclear Accident

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ISBN: 9784431545828 9784431545835 Year: Pages: 223 DOI: 10.1007/978-4-431-54583-5 Language: English
Publisher: Springer Nature
Subject: Nuclear Physics --- Environmental Sciences
Added to DOAB on : 2014-03-05 12:56:57
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This book provides comprehensive research findings related to the environmental monitoring of radiation, levels of radioactive nuclides in various environments and dose estimation in residents after the Fukushima nuclear power plant accident caused severe environmental contamination with radioactive nuclides. At the beginning of the book, a technical review written by a leading researcher of nuclear reactor technology explains what happened at the power plant. The review is followed by a commentary from a former member of the International Commission on Radiological Protection, providing the reader with easily understandable information about the concept of radiation dosage. In the main part of the book, a series of scientific reports presents valuable data on the radiation surveys of the environment, environmental radioactivity, transfer models and parameters of radioactive nuclides and dose assessment among residents. These reports present a wide range of findings from the research carried out in a variety of activities by large governmental organizations as well as by small private groups and individuals. The reader thus will find a large collection of valuable and interesting data related to the environmental contamination by radioactive nuclides after the Fukushima accident. Although earlier reports on this issue have been made public, this book is the only publication to fully depict the actual situation by providing comprehensive data obtained by diverse organizations and individuals.

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