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Fluorescence Probes for Sensing

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ISBN: 9783038429272 9783038429289 Year: Pages: VIII, 306 Language: English
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Environmental Sciences
Added to DOAB on : 2018-05-08 13:04:30
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The rapid progress in sensor science in recent years has resulted in the development of fluorescence probes with enhanced analytical capabilities. Because of the vast evolution of this research field, therefore, we have decided to combine all the research articles published in "Fluorescence Probes and Sensors" for a Special Issue (SI) book of Sensors which was focusing on the important role sensors play in “Fluorescent Probes and Sensors”. Fluorescence novel Probes make an ideal candidate for promising applications in biological analytes and environmental monitoring. Fluorescent probes along with metal complexes have been developed as a new class of fluorophores with excellent properties. This book illustrating the suitability of newly developed sensors for fluorescent analysis applications, as well as describing novel applications of established sensors in solving real life analytical problems.

Gas transfer at the air-water interface in a turbulent flow environment

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Book Series: Dissertationsreihe am Institut für Hydromechanik der Universität Karlsruhe (TH) ISSN: 14394111 ISBN: 3937300740 Year: Volume: 2005,4 Pages: IX, 128 p. DOI: 10.5445/KSP/1000003544 Language: ENGLISH
Publisher: KIT Scientific Publishing
Subject: General and Civil Engineering
Added to DOAB on : 2019-07-30 20:01:58
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The gas transfer process across the air-water interface in a ottom-shear-induced turbulent environment was investigated to gain improved fundamental understanding of the physical mechanisms that control the process. For this purpose, it is necessary to reveal the hydrodynamics of the flow field as well as the molecular diffusion and the turbulent transport contributions to the total flux. Therefore, detailed laboratory experiments were conducted to obtain these information.The experiments were performed in a grid-stirred tank using a combined Particle Image Velocimetry - Laser Induced Fluorescence (PIV-LIF) technique that has been developed for these near surface gas transfer measurements. The turbulence characteristics of the velocity near the interface were acquired from the PIV measurements and showed generally good agreement with the theoretical profiles from Hunt & Graham (1978). The LIF technique enabled visualization of the planar concentration fields which provided more insight into the gas transfer mechanisms. The high data resolution allowed detailed quantification of the concentration distribution within the thin aqueous boundary layer. The mean and turbulent fluctuation characteristics of the concentration could be elucidated and the molecular diffusion contribution to the total flux across the interface could be determined. With the combined PIV-LIF technique, which enables simultaneous and spatially synoptic measurements of 2D velocity and concentration fields, the turbulent mass flux term cw and also the total mass flux across the air-water interface could be quantified directly. For the first time, a particular trend can be inferred from the measured mean cw profiles. It could also be shown that the contribution of the turbulent mass flux to the total gas flux is significant. The co-spectra indicated different behavior for the cases with lower and higher turbulent Reynolds numbers.The interrelated interpretation of the obtained results suggest that the gas transfer process is controlled by a spectrum of different eddy sizes and the gas transfer at different turbulence levels can be associated to certain eddy sizes. For high turbulence levels the gas transfer should be asymptotic to the small eddy model, whereas for low turbulence level to the large eddy model. The new results of turbulent mass flux should aid as an excellent database in refining numerical models and developing more accurate models for the prediction of the transfer velocity.

Replication-Competent Reporter-Expressing Viruses

ISBN: 9783038422587 9783038422594 Year: Pages: XVI, 322 Language: English
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Biology
Added to DOAB on : 2016-10-31 16:58:45
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Recombinant viruses expressing reporter fluorescent or bioluminescent proteins are an excellent option to evaluate the dynamics of viral infection progression in both cultured cells and/or validated animal models of viral infection. Reporter proteins are valid surrogates for direct detection of infected cells in vitro and in vivo, without the use of secondary methodologies to identify infected cells. By eliminating the need of secondary labeling, tractable replicating-competent, reporter-expressing viruses provide an ideal approach to monitor viral infections in real time, representing a significant advance in the study of the biology of viruses, to evaluate vaccination approaches, and to identify new therapeutics against viral infections using high-throughput screening settings. In this Special Issue “Replication-Competent Reporter-Expressing Viruses” we review replication-competent, reporter-expressing viruses belonging to different families, methods of characterization, and applications to facilitate the study of in vitro and in vivo viral infections. We also seek to discuss disadvantages and limitations associated with these reporter-expressing viruses. Finally, we provide rational future perspectives and additional avenues for the development, characterization, and applications of recombinant, reporter-expressing, competent viruses.

Condensed Matter Researches in Cryospheric Science

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ISBN: 9783039213238 / 9783039213245 Year: Pages: 144 DOI: 10.3390/books978-3-03921-324-5 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Environmental Sciences
Added to DOAB on : 2019-12-09 16:10:12
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The cryosphere is very sensitive to climate change, and glaciers represent one of the most important archives of atmospheric composition and its variability. From the Himalaya to the European Alps, the longest mid-latitude mountain chain in the world, lie thousands of glaciers that have collected atmospheric compounds over the last millennia. China and Italy are located at the opposite terminals of this long mountain chain, comprising strategic positions for understanding climate evolution and providing important information for the modeling of future climates. The results presented are highlights of some of the most recent advances in cryospheric studies, especially on the topic of mineral dust and aerosols in the atmosphere. They evidence the complexity of the chemical–physical processes involving solid compounds occurring in glacier, snow, and permafrost environments, covering different aspects such as spatial and temporal trends, as well as the impact of mineral and nonmineral particles. Results also show that recent advances in measurement techniques and source apportionment may be powerful and sophisticated tools to provide novel, high-quality scientific information.

Analysis of Peptides and Proteins by Electrophoretic Techniques

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ISBN: 9783039212279 / 9783039212286 Year: Pages: 110 DOI: 10.3390/books978-3-03921-228-6 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Chemistry (General)
Added to DOAB on : 2019-08-28 11:21:27
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The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have significantly increased. The development of microdevices has reduced sample consumption and waste production while high-sensitivity detectors, such as mass spectrometry (MS) or laser-induced fluorescence (LIF), have significantly improved with regards to separation efficiency and detection limits. All of these advancements have led to appreciably enlarged fields of application. Nowadays, a multitude of studies using separation techniques based on electromigration to study proteins and peptides from numerous real matrices are available in the literature. This Special Issue covers the most recent knowledge and advances in the study of peptides and proteins using several electrophoresis techniques, as well as the characterization of relevant proteins and peptides in application areas such as clinical studies, functional foods, and toxicology.

Plasmonics and its Applications

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ISBN: 9783038979142 / 9783038979159 Year: Pages: 196 DOI: 10.3390/books978-3-03897-915-9 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Technology (General)
Added to DOAB on : 2019-06-26 08:44:06
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Plasmonics is a rapidly developing field that combines fundamental research and applications ranging from areas such as physics to engineering, chemistry, biology, medicine, food sciences, and the environmental sciences. Plasmonics appeared in the 1950s with the discovery of surface plasmon polaritons. Plasmonics then went through a novel propulsion in the mid-1970s, when surface-enhanced Raman scattering was discovered. Nevertheless, it is in this last decade that a very significant explosion of plasmonics and its applications has occurred. Thus, this book provides a snapshot of the current advances in these various areas of plasmonics and its applications, such as engineering, sensing, surface-enhanced fluorescence, catalysis, and photovoltaic devices.

Optical MEMS

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ISBN: 9783039213030 / 9783039213047 Year: Pages: 172 DOI: 10.3390/books978-3-03921-304-7 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Technology (General) --- General and Civil Engineering
Added to DOAB on : 2019-12-09 11:49:15
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Optical microelectromechanical systems (MEMS), microoptoelectromechanical systems (MOEMS), or optical microsystems are devices or systems that interact with light through actuation or sensing at a micro- or millimeter scale. Optical MEMS have had enormous commercial success in projectors, displays, and fiberoptic communications. The best-known example is Texas Instruments’ digital micromirror devices (DMDs). The development of optical MEMS was impeded seriously by the Telecom Bubble in 2000. Fortunately, DMDs grew their market size even in that economy downturn. Meanwhile, in the last one and half decade, the optical MEMS market has been slowly but steadily recovering. During this time, the major technological change was the shift of thin-film polysilicon microstructures to single-crystal–silicon microsructures. Especially in the last few years, cloud data centers are demanding large-port optical cross connects (OXCs) and autonomous driving looks for miniature LiDAR, and virtual reality/augmented reality (VR/AR) demands tiny optical scanners. This is a new wave of opportunities for optical MEMS. Furthermore, several research institutes around the world have been developing MOEMS devices for extreme applications (very fine tailoring of light beam in terms of phase, intensity, or wavelength) and/or extreme environments (vacuum, cryogenic temperatures) for many years. Accordingly, this Special Issue seeks to showcase research papers, short communications, and review articles that focus on (1) novel design, fabrication, control, and modeling of optical MEMS devices based on all kinds of actuation/sensing mechanisms; and (2) new developments of applying optical MEMS devices of any kind in consumer electronics, optical communications, industry, biology, medicine, agriculture, physics, astronomy, space, or defense.

Keywords

scanning micromirror --- electromagnetic actuator --- angle sensor --- flame retardant 4 (FR4) --- variable optical attenuator (VOA) --- wavelength dependent loss (WDL) --- polarization dependent loss (PDL) --- micro-electro-mechanical systems (MEMS) --- tunable fiber laser --- echelle grating --- DMD chip --- MEMS scanning micromirror --- fringe projection --- laser stripe scanning --- quality map --- large reflection variations --- 3D measurement --- laser stripe width --- vibration noise --- MLSSP --- MEMS scanning mirror --- wavefront sensing --- digital micromirror device --- ocular aberrations --- dual-mode liquid-crystal (LC) device --- infrared Fabry–Perot (FP) filtering --- LC micro-lenses controlled electrically --- spectrometer --- infrared --- digital micromirror device (DMD) --- signal-to-noise ratio (SNR) --- stray light --- programmable spectral filter --- digital micromirror device --- optical switch --- microscanner --- input shaping --- open-loop control --- quasistatic actuation --- residual oscillation --- usable scan range --- higher-order modes --- resonant MEMS scanner --- electrostatic --- parametric resonance --- NIR fluorescence --- intraoperative microscope --- 2D Lissajous --- fluorescence confocal --- metasurface --- metalens --- field of view (FOV) --- achromatic --- Huygens’ metalens --- bio-optical imaging --- optical coherence tomography --- confocal --- two-photon --- spectrometer --- MEMS mirror --- electrothermal bimorph --- Cu/W bimorph --- electrothermal actuation --- reliability --- n/a

Biological and Biogenic Crystallization

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ISBN: 9783038975212 Year: Pages: 106 DOI: 10.3390/books978-3-03897-522-9 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Biology
Added to DOAB on : 2019-03-08 11:42:05
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The intention of the Special Issue ""Biological and Biogenic Crystallization"" was to create an international platform aimed at covering a broad field of results involving the crystallization of biological molecules, including virus and protein crystallization, biogenic crystallization including physiological and pathological crystallization taking place in living organisms (human beings, animals, plants, bacteria, etc.), and bio-inspired crystallization. Despite many years of research on biological and biogenic crystals, there are still open questions as well as hot and timely topics. This Special Issue contains seven articles that present a cross-section of the current research activities in the of field of biological and biogenic crystals. The authors of the presented articles prove the vibrant and topical nature of this field. We hope that this Special Issue will serve as a source of inspiration for future investigations, and will be useful for scientists and researchers who work on the exploration of biological and biogenic crystals.

Abiotic Stress Effects on Performance of Horticultural Crops

Authors: ---
ISBN: 9783039217502 / 9783039217519 Year: Volume: 1 Pages: 126 DOI: 10.3390/books978-3-03921-751-9 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Biology --- Agriculture (General)
Added to DOAB on : 2019-11-05 10:43:33
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Horticultural crop yield and quality depend on genotype, environmental conditions, and production management. In particular, adverse environmental conditions may greatly affect crop performance, reducing crop yield by 50%–70%. Abiotic stresses such as cold, heat, drought, flooding, salinity, nutrient deficiency, and ultraviolet radiation affect multiple physiological and biochemical mechanisms in plants as they attempt to cope with the stress conditions. However, different crop species can have different sensitivities or tolerances to specific abiotic stresses. Tolerant plants may activate different strategies to adapt to or avoid the negative effect of abiotic stresses. At the physiological level, photosynthetic activity and light-use efficiency of plants may be modulated to enhance tolerance against the stress. At the biochemical level, several antioxidant systems may be activated, and many enzymes may produce stress-related metabolites to help avoid cellular damage, including compounds such as proline, glycine betaine, and amino acids. Within each crop species there is a wide variability of tolerance to abiotic stresses, and some wild relatives may carry useful traits for enhancing the tolerance to abiotic stresses in their progeny through either traditional or biotechnological breeding. The research papers and reviews presented in this book provide an update of the scientific knowledge of crop interactions with abiotic stresses.

Hollow core optical fibers

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ISBN: 9783039210886 / 9783039210893 Year: Pages: 182 DOI: 10.3390/books978-3-03921-089-3 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Technology (General) --- General and Civil Engineering
Added to DOAB on : 2019-08-28 11:21:27
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The possibility of guiding light in air has fascinated optical scientists and engineers since the dawn of optical fiber technology. In the last few years, hollow core optical fibers have been attracting the attention of an expanding worldwide research community, furthering the design, fabrication and device implementation of specialty optical fibers. Hollow core optical fibers are entering almost any specific application field of optics from medicine to security; from telecommunication to industrial processing; from instrumentation to biology. In parallel to the increased number of applications, major advances are still being made on the optimization of hollow core fiber designs and on the study of its underlying guiding properties, as well as in the use of different materials and fabrication techniques, which, in turn, are providing even more ways of exploitation of this technology and new technical challenges. This Special Issue of Fibers rides the wave of this increasing interest in the field of hollow core optical fibers by providing an overview of the recent progress in this field as well as an updated and indicative sample of current research activities worldwide.

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