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Chapter: 'Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System' from book: 3D Cell Culture: Methods and Protocols (Book chapter)

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Book Series: Methods in Molecular Biology ISSN: 1064-3745 / 1940-6029 ISBN: 9781493970193 9781493970216 Year: Volume: 1612 Pages: 13 DOI: https://doi.org/10.1007/9781493970216_15 Language: English
Publisher: Springer Nature
Subject: Oncology
Added to DOAB on : 2017-07-19 15:44:38
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Metastasis is the main cause of cancer patient mortality. Local tumor invasion is a key step in metastatic dissemination whereby cancer cells dislodge from primary tumors, migrate through the peritumoral stroma and reach the circulation. This is a highly dynamic process occurring in three dimensions that involves interactions between tumor, stromal cells, and the extracellular matrix. Here we describe the organotypic culture system and its utility to study breast cancer cell invasion induced by cancer-associated fibroblasts. This is a three-dimensional model that reproduces the biochemical and physiological properties of real tissue and allows for investigating the molecular and cellular mechanisms involving tumor and its microenvironment, and their contribution to cancer cell invasion. This system provides a robust, accurate, and reproducible method for measuring cancer cell invasion and represents a valuable tool to improve the mechanistic understanding of the initial steps in metastasis.

Plant Competition in a Changing World

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Book Series: Frontiers Research Topics ISSN: 16648714 ISBN: 9782889452057 Year: Pages: 154 DOI: 10.3389/978-2-88945-205-7 Language: English
Publisher: Frontiers Media SA
Subject: Botany --- Science (General)
Added to DOAB on : 2017-10-13 14:57:01
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Competitiveness describes a key ability important for plants to grow and survive abiotic and biotic stresses. Under optimal, but particularly under non-optimal conditions, plants compete for resources including nutrients, light, water, space, pollinators and other. Competition occurs above- and belowground. In resource-poor habitats, competition is generally considered to be more pronounced than in resource-rich habitats. Although competition occurs between different players within an ecosystem such as between plants and soil microorganisms, our topic focusses on plant-plant interactions and includes inter-specific competition between different species of similar and different life forms and intra-specific competition.Strategies for securing resources via spatial or temporal separation and different resource needs generally reduce competition. Increasingly important is the effect of invasive plants and subsequent decline in biodiversity and ecosystem function. Current knowledge and future climate predictions suggest that in some situations competition will be intensified with occurrence of increased abiotic (e.g. water and nutrient limitations) and biotic stresses (e.g. mass outbreak of insects), but competition might also decrease in situations where plant productivity and survival declines (e.g. habitats with degraded soils).Changing interactions, climate change and biological invasions place new challenges on ecosystems. Understanding processes and mechanisms that underlie the interactions between plants and environmental factors will aid predictions and intervention. There is much need to develop strategies to secure ecosystem services via primary productivity and to prevent the continued loss of biodiversity.This Research Topic provides an up-to-date account of knowledge on plant-plant interactions with a focus on identifying the mechanisms underpinning competitive ability. The Research Topic aims to showcase knowledge that links ecological relevance with physiological processes to better understanding plant and ecosystem function.

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