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Toxicology - New Aspects to This Scientific Conundrum

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ISBN: 9789535127161 9789535127178 Year: Pages: 218 DOI: 10.5772/62600 Language: English
Publisher: IntechOpen
Subject: Public Health
Added to DOAB on : 2019-10-03 07:51:49

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This edited book, Toxicology - New Aspects to This Scientific Conundrum, is intended to provide an overview on the different xenobiotics employed every day in our anthropogenic activities. We hope that this book will continue to meet the expectations and needs of all interested in the implications for the living species of known and new toxicants and to guide them in the future investigations.

Ecological Risk Assessment for Chlorpyrifos in Terrestrial and Aquatic Systems in North America

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Book Series: Reviews of Environmental Contamination and Toxicology ISSN: 21976554 ISBN: 9783319038643 9783319038650 Year: Volume: 231 Pages: 282 DOI: 10.1007/978-3-319-03865-0 Language: English
Publisher: Springer
Subject: Biotechnology --- Environmental Sciences --- Manufactures --- Pharmacy and materia medica
Added to DOAB on : 2014-07-25 15:51:35
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This book attempts to provide concise, critical reviews of timely advances, philosophy and significant areas of accomplished or needed endeavor in the total field of xenobiotics, in any segment of the environment, as well as toxicological implications.

30 years of the Comet Assay: an overview with some new insights

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Book Series: Frontiers Research Topics ISSN: 16648714 ISBN: 9782889196494 Year: Pages: 174 DOI: 10.3389/978-2-88919-649-4 Language: English
Publisher: Frontiers Media SA
Subject: Science (General) --- Genetics
Added to DOAB on : 2016-08-16 10:34:25
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By means of this ‘Frontiers in Genetics’ research topic, we are celebrating 30 years of the Comet Assay. The first paper on this single-cell gel electrophoresis assay was published in 1984 by O. Ostling and K.J. Johanson (Biochem. Biophys. Res. Commun. Vol.123: 291-298). The comet assay is a versatile and sensitive method for measuring single - and double-strand breaks in DNA. By including lesion-specific enzymes in the assay, its range and sensitivity are greatly increased, but it is important to bear in mind that their specificity is not absolute. The comet assay (with and without inclusion of lesion-specific enzymes) is widely used as a biomarker assay in human population studies - primarily to measure DNA damage, but increasingly also to assess the capacity of cells for DNA repair. Ostling and Johanson (Biochem. Biophys. Res. Commun., 1984) were also the first to report experiments to measure DNA repair, by simply following the decrease of DNA damage over time after challenging cells with ionising radiation. However, this approach is time-consuming and laborious as it requires an extended period of cell culture and is therefore not ideal for biomonitoring studies, which typically require high-throughput processing of many samples. As an alternative approach, the in vitro comet-based repair assay was developed: a cell extract is incubated with a DNA substrate containing specific lesions, and DNA incisions accumulate. The in vitro comet-based repair assay has been modified and improved over the past decade: it was first devised to measure base excision repair of oxidised purines in lymphocytes (Collins et al., Mutagenesis, 2001), but has since been adapted for other lesions and thus other repair pathways, as well as being applied to tissue samples in addition to cell suspensions. Even after 30 years, the comet assay is still in a growth phase, with many new users each year. Many questions are repeatedly raised, which may seem to have self-evident answers, but clearly, it is necessary to reiterate them for the benefit of the new audience, and sometimes being forced to think again about old topics can shed new light. Different applications of the comet assay are discussed in this special issue, including: genotoxicity testing in different organisms, human biomonitoring, DNA repair studies, environmental biomonitoring and clinical studies. Furthermore, we consider and where possible answer questions, including the ones raised by Raymond Tice at the 8th International Comet Assay Workshop in Perugia (Italy 2009): What is the spectrum of DNA damage detected by the various versions of the comet assay?; What are the limitations associated with each application?; What should be done to standardize the assay for biomonitoring studies?; Can the comet assay be used to monitor changes in global methylation status?; What cell types are suitable for detecting genotoxic substances and their effects in vivo and in vitro?; Can the assay be fully automated?; and more. So this ‘Frontiers in Genetics’ research topic is written for the beginner as well as for the experienced users of the comet assay.

Geomicrobiology and Biogeochemistry of Precious Metals

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ISBN: 9783038973461 9783038973478 Year: Pages: 182 DOI: 10.3390/books978-3-03897-347-8 Language: English
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Biotechnology --- Ecology --- Geology --- Earth Sciences --- Environmental Sciences
Added to DOAB on : 2018-11-20 10:57:53
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Precious metals continue to have economic and sociocultural importance, as their usage evolves and diversifies over time. Today, the industrial application of precious metals is increasing with the development of scientific and technological innovations. Especially, the biological cycling of these metals is receiving more and more attention, as the microbiota may be key to a range of issues regarding exploration, ore-processing and metallurgy, and the processing of electron waste. In this volume, we focus on enhancing the fundamental understanding of the biological processes that drive noble metal cycling and examine how this knowledge may be turned into biotechnolical applications.

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