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Recombinant protein expression in microbial systems

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Book Series: Frontiers Research Topics ISSN: 16648714 ISBN: 9782889192946 Year: Pages: 102 DOI: 10.3389/978-2-88919-294-6 Language: English
Publisher: Frontiers Media SA
Subject: Environmental Sciences --- Biotechnology --- General and Civil Engineering --- Microbiology --- Science (General)
Added to DOAB on : 2015-12-10 11:59:07
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Abstract

With the advent of recombinant DNA technology, expressing heterologous proteins in microorganisms rapidly became the method of choice for their production at laboratory and industrial scale. Bacteria, yeasts and other hosts can be grown to high biomass levels efficiently and inexpensively. Obtaining high yields of recombinant proteins from this material was only feasible thanks to constant research on microbial genetics and physiology that led to novel strains, plasmids and cultivation strategies. Despite the spectacular expansion of the field, there is still much room for progress. Improving the levels of expression and the solubility of a recombinant protein can be quite challenging. Accumulation of the product in the cell can lead to stress responses which affect cell growth. Buildup of insoluble and biologically inactive aggregates (inclusion bodies) lowers the yield of production. This is particularly true for obtaining membrane proteins or high-molecular weight and multi-domain proteins. Also, obtaining eukaryotic proteins in a prokaryotic background (for example, plant or animal proteins in bacteria) results in a product that lack post-translational modifications, often required for functionality. Changing to a eukaryotic host (yeasts or filamentous fungi) may not be a proper solution since the pattern of sugar modifications is different than in higher eukaryotes. Still, many advances in the last couple of decades have provided to researchers a wide variety of strategies to maximize the production of their recombinant protein of choice. Everything starts with the careful selection of the host. Be it bacteria or yeast, a broad list of strains is available for overcoming codon use bias, incorrect disulfide bond formation, protein toxicity and lack of post-translational modifications. Also, a huge catalog of plasmids allows choosing for different fusion partners for improving solubility, protein secretion, chaperone co-expression, antibiotic resistance and promoter strength. Next, controlling culture conditions like temperature, inducer and media composition can bolster recombinant protein production. With this Research Topic, we aim to provide an encyclopedic account of the existing approaches to the expression of recombinant proteins in microorganisms, highlight recent discoveries and analyze the future prospects of this exciting and ever-growing field.

New Approaches for the Discovery of Pharmacologically-Active Natural Compounds

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ISBN: 9783039211043 9783039211050 Year: Pages: 158 DOI: 10.3390/books978-3-03921-105-0 Language: English
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Medicine (General) --- Therapeutics
Added to DOAB on : 2019-08-28 11:21:27
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A major source of active compounds, natural products from different sources supply a large variety of molecules that have been approved for clinical use or used as the starting points of optimization programs. This book features nine papers (eight full articles and one review paper) written by more than 45 scientists from around the world. These papers illustrate the development and application of a broad range of computational and experimental techniques applied to natural product research. On behalf of the contributors to the book, our hope is that the research presented here contributes to advancements in the field, and encourages multidisciplinary teams, young scientists, and students to further advance in the discovery of pharmacologically-active natural compounds

Promising Detoxification Strategies to Mitigate Mycotoxins in Food and Feed

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ISBN: 9783038970286 9783038970279 Year: Pages: 306 DOI: 10.3390/books978-3-03897-027-9 Language: English
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Medicine (General) --- Public Health
Added to DOAB on : 2019-08-28 11:21:28
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This book is a printed edition of the Special Issue Promising Detoxification Strategies to Mitigate Mycotoxins in Food and Feed that was published in Toxins

Keywords

deoxynivalenol --- epimer --- polarity --- Tri101 --- molecular --- interactions --- spores of Ganoderma lucidum --- oxidative stress --- aflatoxins --- antioxidant capability --- mycotoxin --- patulin --- biodegradation --- Pichia caribbica --- proteomics --- intracellular and extracellular enzymes --- Bacillus licheniformis CK1 --- zearalenone (ZEA) --- serum hormones --- estrogen receptor (ER) --- post-weaning female piglets --- curcumin --- aflatoxin B1 --- CYP450 --- AFBO–DNA --- chicks --- aflatoxin B1 --- photodegradation product --- TQEF-MS/MS --- cell viability --- furan rings --- mycotoxin --- toxigenic Fusarium --- biological control --- Trichoderma --- modified mycotoxin --- aflatoxin B1 --- aflatoxin biodegradation preparation --- Bacillus subtilis ANSB060 --- ameliorating effects --- growth performance --- antioxidant function --- residue --- aflatoxins --- biotransformation --- enzymatic detoxification --- laccase --- mild technologies --- food safety --- mycotoxins mitigation --- aflatoxin B1 --- aflatoxin-degrading enzyme --- biodegradation --- Bacillus shackletonii --- purification --- Sporobolomyces sp. IAM 13481 --- microbial patulin degradation --- desoxypatulinic acid --- ascladiol --- aflatoxins --- Aspergillus flavus --- Corylus avellana --- fatty acids --- thermal treatment --- Aflatoxin B1 --- Aspergillus flavus --- hyssop --- inhibition --- oxidative stress --- DBD --- atmospheric pressure --- low temperature plasma --- mycotoxins --- degradation --- maize --- aflatoxins --- neutral electrolyzed water --- detoxification --- turkey --- mycotoxins --- biotransformation --- degradation --- enzymes --- application --- mycotoxin --- detoxification --- biodegradation --- biotransformation --- enzyme --- microorganism identification --- mycotoxin --- trichothecene --- deoxynivalenol --- bioprospecting --- detoxification --- Fusarium --- cold atmospheric pressure plasma technology --- mycotoxins --- physical decontamination --- chemical decontamination --- biological decontamination --- patulin --- mycotoxin --- mitigation --- decontamination --- food and beverage --- processing --- n/a

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