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Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice

Authors: --- --- --- --- et al.
ISBN: 9783319773384 Year: Pages: 39 DOI: 10.1007/978-3-319-77338-4 Language: English
Publisher: Springer Nature
Subject: Agriculture (General) --- Botany
Added to DOAB on : 2020-02-04 11:21:03
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This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted – the seedling and flowering stages – with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly. The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance.

Linear Selection Indices in Modern Plant Breeding

Authors: ---
ISBN: 9783319912233 Year: Pages: 256 DOI: 10.1007/978-3-319-91223-3 Language: English
Publisher: Springer Nature
Subject: Zoology --- Botany --- Biology
Added to DOAB on : 2020-02-05 11:21:15
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This open access book focuses on the linear selection index (LSI) theory and its statistical properties. It addresses the single-stage LSI theory by assuming that economic weights are fixed and known - or fixed, but unknown - to predict the net genetic merit in the phenotypic, marker and genomic context. Further, it shows how to combine the LSI theory with the independent culling method to develop the multistage selection index theory. The final two chapters present simulation results and SAS and R codes, respectively, to estimate the parameters and make selections using some of the LSIs described. It is essential reading for plant quantitative geneticists, but is also a valuable resource for animal breeders.

Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols

Authors: --- --- --- --- et al.
ISBN: 9783319162584 9783319162591 Year: Pages: 35 DOI: 10.1007/978-3-319-16259-1 Language: English
Publisher: Springer Nature
Subject: Biotechnology --- Plant Sciences --- Manufactures --- Chemical Engineering --- Pharmacy and materia medica
Added to DOAB on : 2016-04-12 17:07:01
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Open Access provided with a grant from the International Atomic Energy Agency.This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

Biotechnologies for Plant Mutation Breeding: Protocols

Authors: --- --- ---
ISBN: 9783319450193 9783319450216 Year: Pages: 340 DOI: 10.1007/978-3-319-45021-6 Language: English
Publisher: Springer Nature
Subject: Manufactures --- Chemical Engineering --- Biotechnology --- Therapeutics
Added to DOAB on : 2017-03-08 13:33:36
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This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.

Advances in Legume Research

Authors: --- --- ---
Book Series: Frontiers Research Topics ISSN: 16648714 ISBN: 9782889456321 Year: Pages: 609 DOI: 10.3389/978-2-88945-632-1 Language: English
Publisher: Frontiers Media SA
Subject: Science (General) --- Botany
Added to DOAB on : 2019-01-23 14:53:43
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Legumes crops have an extraordinary importance for the agriculture and the environment. In a world urgently requiring more sustainable agriculture, food security and healthier diets the demand for legume crops is on the rise. The International Legume Society (http://ils.nsseme.com) organizes a triannual series of conferences with the goal to serve as a forum to discuss interdisciplinary progress on legume research. The Second International Legume Society Conference (ILS2) hosted in October 2016 at Troia, Portugal was the starting point for the Research Topic “Advances in Legume Research” in FiPS, that was also open to spontaneous submissions.

The Potato Crop

Authors: ---
ISBN: 9783030286835 Year: Pages: 518 DOI: 10.1007/978-3-030-28683-5 Language: English
Publisher: Springer Nature
Subject: Agriculture (General) --- Chemical Technology --- Botany --- Biology
Added to DOAB on : 2020-02-04 11:21:17
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Life sciences; Agriculture; Nutrition   ; Plant breeding; Food—Biotechnology; Agricultural economics

The Brassicaceae Agri-Horticultural and Environmental Perspectives

Authors: --- --- --- --- et al.
Book Series: Frontiers Research Topics ISSN: 16648714 ISBN: 9782889456451 Year: Pages: 369 DOI: 10.3389/978-2-88945-645-1 Language: English
Publisher: Frontiers Media SA
Subject: Science (General) --- Botany
Added to DOAB on : 2019-01-23 14:53:43
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This Frontiers Research Topic "The Brassicaceae- Agri-Horticultural and Environmental Perspectives" is an effort to provide a common platform to agronomists, horticulturists, plant breeders, plant geneticists/molecular biologists, plant physiologists and environmental plant scientists exploring major insights into the role of important members of the plant family Brassicaceae (the mustard family, or Cruciferae) in agri-horticultural and environmental arenas.

Sowing Legume Seeds, Reaping Cash

Authors: --- --- --- --- et al.
ISBN: 9789811508455 Year: Pages: 106 DOI: 10.1007/978-981-15-0845-5 Language: English
Publisher: Springer Nature
Subject: Agriculture (General) --- Botany
Added to DOAB on : 2020-06-17 00:00:58
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This open access book shares impact stories – testimonies from various value chain actors who have been part of the Tropical Legumes (TL) projects, over the past twelve years. The Tropical Legumes projects led by ICRISAT in three parts (TLI, TLII and TLIII), constitute a major international initiative supported by the Bill & Melinda Gates Foundation (BMGF) and jointly implemented by the International Center for Tropical Agriculture (CIAT), International Institute of Tropical Agriculture (IITA) and National Agricultural Research Systems (NARS) partners from Sub-Saharan Africa and India. The project developed improved cultivars of common bean, cowpea, chickpea and groundnut (but also soya bean and pigeon pea cultivars in its initial phases) and delivers their seed to smallholders in BMGF-focus areas. It also strengthens the NARS and CGIAR's breeding programs and seed platforms to enhance their ability to deliver high and sustained outputs to smallholder farmers. The book compiles the experiences of a diversity of actors within the grain legume value chains, with a focus on groundnut and common beans in Tanzania and Uganda, groundnut and cowpea in Nigeria, and groundnut in Ghana. All stakeholders involved share their thoughts on being part of a decade-long development project family. National agricultural research institutes, knowledge brokering organizations, NGOs, public and private seed companies, agro-dealers, individual seed entrepreneurs, farm-implement makers, farmer cooperatives, farmer groups, individual men and women farmers, middlemen, processors, traders and consumers were all involved in this project, and as such this book provides valuable insights for development workers, technical staff, and project managers.

Molecular Genetics, Genomics and Biotechnology of Crop Plants Breeding

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ISBN: 9783039288779 / 9783039288786 Year: Pages: 238 DOI: 10.3390/books978-3-03928-878-6 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Biology --- Plant Sciences
Added to DOAB on : 2020-06-09 16:38:57
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This Special Issue on molecular genetics, genomics, and biotechnology in crop plant breeding seeks to encourage the use of the tools currently available. It features nine research papers that address quality traits, grain yield, and mutations by exploring cytoplasmic male sterility, the delicate control of flowering in rice, the removal of anti-nutritional factors, the use and development of new technologies for non-model species marker technology, site-directed mutagenesis and GMO regulation, genomics selection and genome-wide association studies, how to cope with abiotic stress, and an exploration of fruit trees adapted to harsh environments for breeding purposes. A further four papers review the genetics of pre-harvest spouting, readiness for climate-smart crop development, genomic selection in the breeding of cereal crops, and the large numbers of mutants in straw lignin biosynthesis and deposition.

Keywords

phloem metabolites --- electrospray ionisation --- mass spectrometry --- cultivar --- quality groups --- nitrogen --- faba bean --- zt-1 --- linkage map --- SSR --- ISSR --- Brassica napus --- GmDof4 --- GmDof11 --- oleic acid --- fatty acid composition --- differentially expressed genes --- drought --- RNA-seq --- RNA editing --- wheat --- climate change --- mapping populations --- genetic resources --- mutation breeding --- genome editing --- new plant breeding techniques --- “omics” data --- bioinformatics --- rice --- CRISPR/Cas9 --- Wx --- TGW6 --- mutations --- maintainer --- cytoplasmic male sterile --- amylose content --- anther --- protein --- cytoplasmic male sterility --- fertility restoration --- sunflower --- Rf1 gene --- GWAS --- Pentatricopeptide Repeats --- PPR genes --- association mapping --- candidate genes --- gene mapping --- lodicule --- non-open hull 1(noh1) --- rice --- crops --- quantitative genetics --- estimated breeding value --- genomic prediction --- plant breeding --- breeding scheme --- pedigree --- genetic value --- wheat --- pre-harvest sprouting --- seed dormancy --- abscisic acid --- gibberellin --- QTL/genes --- brown midrib --- cell wall --- gold hull and internode --- grass family --- lignin --- monolignol pathway --- mutational breeding --- orange lemma --- transgenic cereals --- SNP --- SSR --- next generation sequencing --- genotyping by sequencing --- Japanese plum --- SSR --- diversity --- genetic structure --- candidate genes --- genomic selection --- mutants --- ddRAD sequencing --- genotyping-by-sequencing --- CRISPR/Cas9 site directed mutagenesis --- genome-wide association scan --- genetic modification --- F1 hybrids --- QTL

Analysis of Sensory Properties in Foods

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ISBN: 9783039214334 9783039214341 Year: Pages: 132 DOI: 10.3390/books978-3-03921-434-1 Language: English
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Biology --- Nutrition and Food Sciences
Added to DOAB on : 2019-12-09 11:49:15
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The sensory properties of foods are the most important reason people eat the foods they eat. What those properties are and how we best measure those properties are critical to understanding food and eating behavior. Appearance, flavor, texture, and even the sounds of food can impart a desire to eat or cause us to dismiss the food as unappetizing, stale, or even inappropriate from a cultural standpoint. This Special Issue focuses on how sensory properties are measured, the specific sensory properties of various foods, and consumer behavior related to which properties might be most important in certain situations and how consumers use sensory attributes to make decisions about what they will eat. This Special Issue contains both research papers and review articles.

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