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Lectins. Methods and protocols

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ISBN: 9781493912919 Year: DOI: 10.1007/978-1-4939-1292-6 Language: English
Publisher: Springer Grant: FP7 Ideas: European Research Council - 311532
Subject: Biology
Added to DOAB on : 2018-06-22 11:01:50

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Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on “engineer” novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lectins: Methods and Protocols seeks to aid not only lectin specialists but also non-experts including both young scientists.

Looking Forward to the Future of Heparin: New Sources, Developments and Applications

Authors: ---
ISBN: 9783038429494 / 9783038429500 Year: Pages: 282 DOI: 10.3390/books978-3-03842-950-0 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Chemistry (General)
Added to DOAB on : 2019-08-28 11:21:28
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This book is a printed edition of the Special Issue Looking Forward to the Future of Heparin: New Sources, Developments and Applications that was published in Molecules

Keywords

thrombin inhibition --- LMWH --- antithrombin --- heparin oligosaccharides --- ternary complex --- heparin --- hepcidin --- iron homeostasis --- anemia --- heparin-induced thrombocytopenia --- diagnosis --- functional assay --- platelets --- heparin --- heparan sulphate --- TGF-? --- bone morphogenetic protein (BMP) --- growth and differentiation factor (GDF) --- GDNF --- BMP antagonists --- noggin --- sclerostin --- gremlin --- heparin --- enoxaparin --- subarachnoid hemorrhage --- edema --- brain injury --- inflammation --- cisplatin --- low molecular weight heparin (LMWH) --- ovarian cancer --- resistance --- heparin --- glycosaminoglycans --- chondroitin sulfate --- perylene diimide dyes --- dermatan sulfate --- fluorescent probe --- Heparin Red --- assay --- dermatan sulfate --- human plasma --- heparin --- alginate --- sulfated alginate --- biomaterials --- heparin --- heparan sulfate --- serglycin --- proteoglycan --- recombinant expression --- bioreactor --- theranostics --- solid lipid nanoparticles --- iron oxide nanoparticles --- heparin coating --- intestinal lymphatic absorption --- heparin --- heparin process --- manufacturing methods --- industrial --- super paramagnetic iron oxide nanoparticles (SPION) --- hyaluronic acid (HA) --- bovine serum albumin (BSA) --- Fe3O4·DA-BSA/HA --- paclitaxel (PTX) --- magnetic resonance imaging (MRI) --- low-molecular-weight heparin --- dalteparin --- NMR --- LC-MS --- affinity chromatography --- danaparoid sodium --- low molecular weight glycosaminoglycans --- orthogonal multi-analytical methods --- sequence and compositional investigations --- component quantitative analysis --- heparin --- crude heparin --- NMR --- quantitative NMR --- PCA --- chemometric --- HSQC --- bovine heparin --- porcine heparin --- molecular weight --- size exclusion chromatography --- pharmacopeia --- Fondaparinux sodium --- extended physicochemical characterization --- qNMR --- single crystal X-ray structure --- reference standard --- iduronic acid conformation --- Arixtra® --- n/a --- n/a --- n/a

Plant Proteomic Research 2.0

Author:
ISBN: 9783039210626 / 9783039210633 Year: Pages: 594 DOI: 10.3390/books978-3-03921-063-3 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Biology --- Plant Sciences
Added to DOAB on : 2019-06-26 08:44:07
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Advancements in high-throughput “Omics” techniques have revolutionized plant molecular biology research. Proteomics offers one of the best options for the functional analysis of translated regions of the genome, generating a wealth of detailed information regarding the intrinsic mechanisms of plant stress responses. Various proteomic approaches are being exploited extensively for elucidating master regulator proteins which play key roles in stress perception and signaling, and these approaches largely involve gel-based and gel-free techniques, including both label-based and label-free protein quantification. Furthermore, post-translational modifications, subcellular localization, and protein–protein interactions provide deeper insight into protein molecular function. Their diverse applications contribute to the revelation of new insights into plant molecular responses to various biotic and abiotic stressors.

Keywords

Phalaenopsis --- petal --- pollination --- senescence --- 2-DE --- ROS --- Medicago sativa --- leaf cell wall proteome --- cadmium --- quantitative proteomics --- 2D DIGE --- chloroplast --- elevated CO2 --- heat stress --- nucleotide pyrophosphatase/phosphodiesterase --- (phospho)-proteomics --- photosynthesis --- protein phosphorylation --- 14-3-3 proteins --- Oryza sativa L. --- starch --- sucrose --- N utilization efficiency --- proteomics --- 2D --- protein phosphatase --- rice isogenic line --- SnRK1 --- 14-3-3 --- lettuce --- bolting --- proteome --- high temperature --- iTRAQ --- proteome profiling --- iTRAQ --- differentially abundant proteins (DAPs) --- drought stress --- physiological responses --- Zea mays L. --- GS3 --- ? subunit --- heterotrimeric G protein --- mass spectrometric analysis --- RGG3 --- rice --- western blotting --- Dn1-1 --- ?-subunit --- heterotrimeric G protein --- mass spectrometry analysis --- RGG4 --- rice --- western blotting --- Clematis terniflora DC. --- polyphenol oxidase --- virus induced gene silencing --- photosynthesis --- glycolysis --- Camellia sinensis --- chlorotic mutation --- chlorophyll deficiency --- weakening of carbon metabolism --- iTRAQ --- proteomics --- degradome --- wheat --- cultivar --- protease --- papain-like cysteine protease (PLCP) --- subtilase --- metacaspase --- caspase-like --- wheat leaf rust --- Puccinia recondita --- Stagonospora nodorum --- iTRAQ --- proteomics --- somatic embryogenesis --- pyruvate biosynthesis --- Zea mays --- chlorophylls --- LC-MS-based proteomics --- pea (Pisum sativum L.) --- proteome functional annotation --- proteome map --- seeds --- seed proteomics --- late blight disease --- potato proteomics --- Phytophthora infestans --- Sarpo Mira --- early and late disease stages --- Simmondsia chinensis --- cold stress --- proteomics --- leaf --- iTRAQ --- Ricinus communis L. --- cold stress --- seed imbibition --- iTRAQ --- proteomics --- Morus --- organ --- gel-free/label-free proteomics --- flavonoid --- antioxidant activity --- phosphoproteome --- barley --- seed dormancy --- germination --- imbibition --- after-ripening --- sugarcane --- Sporisorium scitamineum --- smut --- proteomics --- RT-qPCR --- ISR --- holm oak --- Quercus ilex --- 2-DE proteomics --- shotgun proteomics --- non-orthodox seed --- population variability --- stresses responses --- ammonium --- Arabidopsis thaliana --- carbon metabolism --- nitrogen metabolism --- nitrate --- proteomics --- root --- secondary metabolism --- proteomics --- wheat --- silver nanoparticles --- plant pathogenesis responses --- data-independent acquisition --- quantitative proteomics --- Pseudomonas syringae --- sweet potato plants infected by SPFMV --- SPV2 and SPVG --- sweet potato plants non-infected by SPFMV --- SPV2 and SPVG --- co-infection --- transcriptome profiling --- gene ontology --- pathway analysis --- lesion mimic mutant --- leaf spot --- phenylpropanoid biosynthesis --- proteomics --- isobaric tags for relative and absolute quantitation (iTRAQ) --- rice --- affinity chromatography --- ergosterol --- fungal perception --- innate immunity --- pattern recognition receptors --- plasma membrane --- proteomics --- proteomics --- maize --- plant-derived smoke --- shoot --- Solanum tuberosum --- patatin --- seed storage proteins --- vegetative storage proteins --- tuber phosphoproteome --- targeted two-dimensional electrophoresis --- B. acuminata petals --- MALDI-TOF/TOF --- GC-TOF-MS --- qRT-PCR --- differential proteins --- n/a

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