Search results: Found 21

Listing 1 - 10 of 21 << page
of 3
>>
Sort by
Lectins. Methods and protocols

Author:
ISBN: 9781493912919 Year: DOI: 10.1007/978-1-4939-1292-6 Language: English
Publisher: Springer Grant: FP7 Ideas: European Research Council - 311532
Subject: Biology
Added to DOAB on : 2018-06-22 11:01:50

Loading...
Export citation

Choose an application

Abstract

Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on “engineer” novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lectins: Methods and Protocols seeks to aid not only lectin specialists but also non-experts including both young scientists.

Drug Metabolism, Pharmacokinetics and Bioanalysis

Authors: ---
ISBN: 9783038979166 / 9783038979173 Year: Pages: 230 DOI: 10.3390/books978-3-03897-917-3 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Medicine (General) --- Therapeutics
Added to DOAB on : 2019-06-26 08:44:06
License:

Loading...
Export citation

Choose an application

Abstract

Drug metabolism/pharmacokinetics and drug interaction studies have been extensively carried out in order to secure the druggability and safety of new chemical entities throughout the development of new drugs. Recently, drug metabolism and transport by phase II drug metabolizing enzymes and drug transporters, respectively, as well as phase I drug metabolizing enzymes, have been studied. A combination of biochemical advances in the function and regulation of drug metabolizing enzymes and automated analytical technologies are revolutionizing drug metabolism research. There are also potential drug–drug interactions with co-administered drugs due to inhibition and/or induction of drug metabolic enzymes and drug transporters. In addition, drug interaction studies have been actively performed to develop substrate cocktails that do not interfere with each other and a simultaneous analytical method of substrate drugs and their metabolites using a tandem mass spectrometer. This Special Issue has the aim of highlighting current progress in drug metabolism/pharmacokinetics, drug interactions, and bioanalysis.

Keywords

procainamide --- N-acetylprocainamide --- ultra-high-pressure liquid chromatography --- rat --- plasma --- pharmacokinetics --- adalimumab --- immunoprecipitation --- liquid chromatography-quadrupole TOF MS --- bioanalysis --- GB3 --- Fabry disease --- LC-QTOF-MS/MS --- B6 --- 129-Glatm1Kul/J --- cytochrome P450 --- drug interaction --- liquid chromatography-tandem mass spectrometry --- organic anion transporting polypeptide --- pharmacokinetics --- Korean red ginseng extract --- metformin --- diabetes --- drug interaction --- pharmacokinetics --- efficacy --- ethyl glucuronide --- hair --- HPLC-MS/MS --- AUDIT score --- alcohol addiction --- eurycomanone --- Eurycoma longifolia --- bioavailability --- pharmacokinetic --- anthraquinone --- glycoside --- aglycone --- LC-MS/MS --- plasma --- protein precipitation --- loxoprofen --- CYP --- UGT --- human liver microsomes --- LC-HR/MS --- mematine --- drug interaction --- liquid chromatography-tandem mass spectrometry --- pharmacokinetics --- biopharmaceuticals --- drying technology --- protein stability --- bioavailability --- pharmacokinetics --- DA-9805 --- saikosaponin a --- paeonol --- imperatorin --- pharmacokinetics --- brain distribution --- Osthenol --- CYP --- UGT --- human liver microsomes --- glucuronidation --- Stauntonia hexaphylla leaf extract --- YRA-1909 --- pharmacokinetics --- chlorogenic acid --- neochlorogenic acid --- cryptochlorogenic acid --- caffeic acid --- caffeic acid O-glucuronides --- LC-MS/MS --- aceclofenac --- diclofenac --- esomeprazole --- pharmacokinetics --- gastric ulcer --- acetyl tributyl citrate --- pharmaceutical excipient --- pharmacokinetics --- metabolic stability --- plasma

Solid Phase Extraction: State of the Art and Future Perspectives

Author:
ISBN: 9783039211586 / 9783039211593 Year: Pages: 176 DOI: 10.3390/books978-3-03921-159-3 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Chemistry (General)
Added to DOAB on : 2019-08-28 11:21:27
License:

Loading...
Export citation

Choose an application

Abstract

This book is a collection of 13 innovative papers describing the state of the art and the future perspectives in solid-phase extraction covering several analytical fields prior to the use of gas or liquid chromatographic analysis. New sorptive materials are presented including carbon nanohorn suprastructures on paper support, melamine sponge functionalized with urea–formaldehyde co-oligomers, chiral metal–organic frameworks, UiO-66-based metal–organic frameworks, and fabric phase sorptive media for various applications. Solid-phase extraction can be applied in several formats aside from the conventional cartridges or mini-column approach, e.g., online solid-phase extraction, dispersive solid-phase microextraction, and in-syringe micro-solid-phase extraction can be very helpful for analyte pre-concentration and sample clean-up. Polycyclic musks in aqueous samples, 8-Nitroguanine in DNA by chemical derivatization antibacterial diterpenes from the roots of salvia prattii, perfluoroalkyl substances (PFASs) in aater samples by bamboo charcoal-based SPE, parabens in environmental water samples, benzotriazoles as environmental pollutants, organochlorine pesticide residues in various fruit juices and water samples and synthetic peptide purification are among the applications cited in this collection. All these outstanding contributions highlight the necessity of this analytical step, present the advantages and disadvantages of each method and focus on the green analytical chemistry guidelines that have to be fulfilled in current analytical practices.

Innovative Extraction Techniques and Hyphenated Instrument Configuration for Complex Matrices Analysis

Authors: --- ---
ISBN: 9783039210848 / 9783039210855 Year: Pages: 308 DOI: 10.3390/books978-3-03921-085-5 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Technology (General) --- Chemical Engineering
Added to DOAB on : 2019-06-26 08:44:06
License:

Loading...
Export citation

Choose an application

Abstract

The present Special Issue, “Innovative Extraction Techniques and Hyphenated Instrument Configuration for Complex Matrices Analysis”, aims to collect and to disseminate some of the most significant and recent contributions in the interdisciplinary area of innovative extraction procedures from complex matrices followed by validated analytical methods using hyphenated instrument configurations to support the optimization of the whole process and the scale-up possibility

Keywords

branched-chain keto acids --- serum --- muscle --- HPLC-Q-TOF/MS --- wood --- odor --- volatile organic compounds --- gas chromatography-olfactometry --- Wuyi Rock tea --- quality --- UPLC-QTOF MS --- UPLC-QqQ MS --- metabolite profiling --- metabolomics --- cluster analysis --- cultivars --- Lycium ruthenicun --- flavonoid --- ruthenicunoid A --- SIRT1 --- Asphodeline lutea --- HPLC-PDA --- heavy metals --- tyrosinase --- diabetes --- neurodegenerative disease --- color analysis --- pigments --- MAE --- HPLC-PDA --- SFE --- Thymus algeriensis --- rare earth elements --- flow injection --- inductively coupled plasma-mass spectrometry --- seawater --- Hericium erinaceuns mycelium --- high-speed counter-current chromatography (HSCCC) --- genistein --- daidzein --- Fe3O4 --- modification --- alginate --- alkaloid --- ultrasound assisted extraction --- gelatin --- actinidin --- bovine hide --- physicochemical properties --- gel strength --- Semen Cuscutae --- ultra-high-performance liquid chromatography coupled to electrospray ionization mass spectrometry --- chlorogenic acids --- flavonoids --- steamed Panax notoginseng --- saponins --- extraction --- optimization --- antioxidant activity --- response surface methodology --- hematopoiesis --- microwave processing --- response surface methodology --- minor ginsenosides --- blood-enriching activity --- hemostatic activity --- ionic liquids --- sample preparation --- microextraction --- solid-phase microextraction --- dispersive liquid-liquid microextraction --- single-drop microextraction --- stir bar sorptive extraction --- stir cake sorptive extraction --- rosuvastatin --- metformin --- HILIC --- LC-MS --- therapeutic drug monitoring --- oolong tea --- Tieguanyin tea cultivar --- metabolite profiling --- UPLC-QTOF MS --- metabolomics --- Ajuga genevensis --- near-infrared spectroscopy --- dry extract --- fluid bed process --- microNIR --- in-line monitoring --- total polyphenolic content --- antioxidant --- flavonoids --- mouse melanoma B16 cells --- Panax notoginseng --- surfactant --- n/a --- ultra-high-performance liquid chromatography tandem mass spectrometry --- GHB --- GHB glucuronide --- nails --- endogenous values --- walnut septum --- polyphenols --- phytosterols --- HPLC-MS/MS --- Ultra-Turrax extraction --- biological activity --- antioxidant activity --- experimental design --- optimization --- phytochemicals

Curcumin in Health and Disease

Author:
ISBN: 9783039214495 / 9783039214501 Year: Pages: 274 DOI: 10.3390/books978-3-03921-450-1 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Chemistry (General)
Added to DOAB on : 2019-12-09 11:49:15
License:

Loading...
Export citation

Choose an application

Abstract

The plant-derived polyphenol curcumin has been used in promoting health and combating disease for thousands of years. Its therapeutic effects have been successfully utilized in Ayurvedic and Traditional Chinese Medicine in order to treat inflammatory diseases. Current results from modern biomolecular research reveal the modulatory effects of curcumin on a variety of signal transduction pathways associated with inflammation and cancer. In this context, curcumin’s antioxidant, anti-inflammatory, anti-tumorigenic, and even anti-metastatic activities are discussed. On the cellular level, the reduced activity of several transcription factors (such as NFkB or AP-1) and the suppression of inflammatory cytokines, matrix degrading enzymes, metastasis related genes and even microRNAs are reported. On functional levels, these molecular effects translate into reduced proliferative, invasive, and metastatic capacity, as well as induced tumor cell apoptosis. All these effects have been observed not only in vitro but also in animal models. In combination with anti-neoplastic drugs like Taxol, kinase inhibitors, and radiation therapy, curcumin potentiates the drugs’ therapeutic power and can protect against undesired side effects. Natural plant-derived compounds like curcumin have one significant advantage: They do not usually cause side effects. This feature qualifies curcumin for primary prevention in healthy persons with a predisposition to cancer, arteriosclerosis, or chronic inflammatory diseases. Nonetheless, curcumin is considered safe, although potential toxic effects stemming from high dosages, long-term intake, and pharmacological interactions with other compounds have yet to be assessed. This Special Issue examines in detail and updates current research on the molecular targets, protective effects, and modes of action of natural plant-derived compounds and their roles in the prevention and treatment of human diseases.

Keywords

brain ischemia --- curcumin --- Alzheimer’s disease --- neurodegeneration --- amyloid --- tau protein --- autophagy --- mitophagy --- apoptosis --- genes --- glioblastoma multiforme --- autophagy --- mitophagy --- curcumin --- chaperone-mediated autophagy --- Akt/mTOR signaling --- transmission electron microscopy --- Curcuma longa --- turmeric tuber --- Zingiberaceae --- TLC bioautography --- antimicrobial agents --- ImageJ --- TLC-MS --- hydrostatic counter-current chromatography --- centrifugal partition chromatography --- curcumin --- death receptor --- apoptosis --- curcumin --- anticancer --- structure activity relationship --- cellular pathway --- mechanism of action --- delivery system --- wound --- wound healing --- diet --- nutrition --- micronutrients --- macronutrients --- curcumin --- amino-acids --- vitamins --- minerals --- curcumin --- oxidative metabolites --- inflamm-aging --- cancer --- metabolic reprogramming --- direct protein binding --- IL-17 --- STAT3 --- SHMT2 --- ageing --- anti-cancer --- autophagy --- microbiota --- senescence --- senolytics --- curcumin --- transthyretin --- amyloidosis --- protein aggregation --- protein misfolding --- drug discovery --- curcumin --- renal cell cancer --- tumor growth --- tumor proliferation --- cell cycling --- curcumin --- reflux esophagitis --- gastroprotection --- gastric ulcer --- Helicobacter pylori --- gastric cancer --- curcumin --- complementary medicine --- cancer treatment --- supportive care --- antioxidants --- anti-inflamation --- ulcerative colitis --- Crohn’s disease --- necrotizing enterocolitis --- curcumin --- inflammatory bowel disease --- curcumin --- silica --- chitosan --- nanoparticles --- anti-tumor --- antioxidant activity --- n/a

Qualitative and Quantitative Analysis of Bioactive Natural Products 2018

Author:
ISBN: 9783038977889 Year: Pages: 374 DOI: 10.3390/books978-3-03897-789-6 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Biology --- Science (General)
Added to DOAB on : 2019-04-05 10:34:31
License:

Loading...
Export citation

Choose an application

Abstract

Throughout most of history, medicinal plants and their active metabolites have represented a valuable source of compounds used to prevent and to cure several diseases. Interest in natural compounds is still high as they represent a source of novel biologically/pharmacologically active compounds. Due to their high structural diversity and complexity, they are interesting structural scaffolds that can offer promising candidates for the study of new drugs, functional foods, and food additives.Plant extracts are a highly complex mixture of compounds and qualitative and quantitative analyses are necessary to ensure their quality. Furthermore, greener methods of extraction and analysis are needed today.This book is based on articles submitted for publication in the Special Issue entitled “Qualitative and Quantitative Analysis of Bioactive Natural Products” that collected original research and reviews on these topics.

Keywords

Talaromyces pinophilus --- talarodiolide --- macrodiolides --- GC/MS --- secondary metabolites --- anthocyanins --- red cabbage --- recycling preparative high performance liquid chromatography --- stability --- UHPLC-MS/MS --- decursin --- decursinol angelate --- nodakenin --- decursinol --- Ginkgo biloba Extract (GBE) --- chromatogram-bioactivity correlation --- bioactive compounds --- endothelial function --- wine --- volatile compounds --- terrain conditions --- odor-activity values --- SPME-GC/MS --- Bolbostemma paniculatum --- identification --- LTQ-Orbitrap --- UPLC --- multivariate statistical analysis --- cannabidiol --- CBD oil --- terpenes --- hemp seed oil --- GC-MS --- HPLC-Q-Exactive-Orbitrap-MS --- Rossa da inverno sel. Rojo Duro onion cultivar --- geographical origin --- amino acids content --- HPLC analysis --- statistical evaluations --- food traceability --- aleuritolic acid --- autophagy --- apoptosis --- HPLC --- Curcuma longa --- turmeric --- curcuminoids --- turmerone --- quantification --- Quercus acuta leaf --- antioxidant --- antibacterial activity --- Staphylococcus aureus --- Erigeron breviscapus extract --- UPLC-ESI-MS/MS --- cerebral ischemia reperfusion injury --- scutellarin --- scutellarein --- HPLC --- Podospermum --- Scorzonera --- triterpenes --- ginseng berry extract --- ginsenosides --- stereoselective and simultaneous analysis --- pharmacokinetics --- oral administration --- saffron --- crocins --- UHPLC analysis --- separation optimisation --- artificial neural network --- response surface methodology --- bioactive natural compounds --- secondary metabolites --- Alzheimer’s disease --- Olea europaea L. --- liquid chromatography --- gas chromatography --- mass spectrometry --- secondary metabolites --- olive leaves --- Moroccan region --- phenolic compounds --- liquid chromatography-mass spectrometry --- chemometrics --- metabolic profiling --- Sorbus --- fruit powders --- phenolic compounds --- carotenoids --- sugars --- organic acids --- chili --- capsaicinoids --- phenolics --- free radical-scavenging --- geographical variation --- 1-triacontanol --- HPLC-ELSD --- biostimulant --- method validation --- Myristica fragrans --- nutmeg --- essential oil --- ionic liquids --- hydrodistillation --- MODDE experimental design --- rice --- phenolic compounds --- phenolic acids --- flavonoids --- anthocyanins --- proanthocyanidins --- antioxidant activity --- extraction --- HPLC methods --- phenolic compounds --- Spondias spp. --- UPLC-MS --- antioxidant capacity --- Iris lactea Pall. var. chinensis (Fisch.) Koidz. --- HPLC-Q-TOF-MS/MS --- qualitative analysis --- quantitative analysis --- C-glycosylflavone --- traditional Chinese medicine decoction --- quality evaluation --- UPLC-QTOF-MS --- UFLC-QQQ-MS --- ShenFu prescription decoction

Modern Sample Preparation Approaches for Separation Science

Author:
ISBN: 9783039214112 / 9783039214129 Year: Pages: 282 DOI: 10.3390/books978-3-03921-412-9 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Chemistry (General) --- Analytical Chemistry
Added to DOAB on : 2019-12-09 11:49:15
License:

Loading...
Export citation

Choose an application

Abstract

This book will provide the most recent knowledge and advances in Sample Preparation Techniques for Separation Science. Everyone working in a laboratory must be familiar with the basis of these technologies, and they often involve elaborate and time-consuming procedures that can take up to 80% of the total analysis time. Sample preparation is an essential step in most of the analytical methods for environmental and biomedical analysis, since the target analytes are often not detected in their in-situ forms, or the results are distorted by interfering species. In the past decade, modern sample preparation techniques have aimed to comply with green analytical chemistry principles, leading to simplification, miniaturization, easy manipulation of the analytical devices, low costs, strong reduction or absence of toxic organic solvents, as well as low sample volume requirements.Modern Sample Preparation Approaches for Separation Science also provides an invaluable reference tool for analytical chemists in the chemical, biological, pharmaceutical, environmental, and forensic sciences.

Keywords

vitamins --- extraction --- determination --- review --- sample preparation --- matrix solid phase dispersion --- sorbent --- miniaturization --- on-line --- blueberry --- non-anthocyanin polyphenol --- vortex-assisted dispersive liquid-liquid microextraction --- response surface methodology --- desirability function approach --- nail --- curie temperature --- high-frequency heating --- liquid chromatography–tandem mass spectrometry --- caffeine --- amlodipine --- gas chromatography --- hydrogel --- hormones --- pectin --- polyvinyl alcohol --- sample preparation --- in-tube SPME --- UHPLC-MS/MS --- organic-based monoliths --- antipsychotics --- plasma samples --- schizophrenic’ patients --- salting-out assisted liquid–liquid extraction --- sugaring-out assisted liquid–liquid extraction --- hydrophobic-solvent assisted liquid–liquid extraction --- subzero-temperature assisted liquid–liquid extraction --- phenolic compounds --- sorbent-based techniques --- multi-spheres adsorptive micro-extraction (MSA?E) --- floating sampling technology --- caffeine and acetaminophen tracers --- environmental water matrices --- vortex-assisted dispersive liquid-liquid microextraction --- China herbal tea --- pesticides residue --- aflatoxins --- UPLC-MS/MS --- vortex-synchronized matrix solid-phase dispersion --- crab shells --- ionic liquids --- anthraquinones --- Cassiae Semen --- sample preparation --- nanocomposite --- pathogenic --- enrichment --- nucleic acid isolation --- sample preparation with TLC/HPTLC --- solvent front position extraction --- solvent delivery with a moving pipette --- automation --- LC–MS/MS --- environmental analysis --- whole water --- trace analysis --- SPE --- large volume --- in-line filter --- sand --- flow rate --- pharmaceuticals --- hormones --- pesticides --- space instrumentation --- liquid chromatography --- oligopeptides --- trapping system --- membrane-based microextraction --- barbiturates --- simultaneous determination --- whole blood --- urine --- liver --- sample preparation --- oxylipins --- protein precipitation --- liquid–liquid extraction --- solid-phase extraction --- biological samples --- chlorophenoxy acid herbicides --- HPLC --- hydrophobic in-tube solid-phase microextraction --- poly (OMA-co-TRIM) monolithic column --- rice grains --- gold --- sample preparation --- preconcentration --- geological samples

Environmental Fate of Emerging Organic Micro-Contaminants

Authors: ---
ISBN: 9783039213672 / 9783039213689 Year: Pages: 128 DOI: 10.3390/books978-3-03921-368-9 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Environmental Sciences
Added to DOAB on : 2019-12-09 11:49:15
License:

Loading...
Export citation

Choose an application

Abstract

The toxicity and fate of pharmaceuticals and other emerging micro-organic contaminants in the natural and built environments have been the focus of much research over the last twenty years. Particular focus has recently centred on the fate of antimicrobial chemicals, including antibiotics and antifungals. The occurrence of such chemicals in the environment is thought to contribute to the selection of resistance in exposed microorganisms.This Special Issue (SI) brings together a broad range of recent advances in the field of emerging micro-organic contaminants, ranging from medicinal contaminants to industrial chemicals in the environment. Notably, these range from chemical extraction and large-scale analysis to adverse effects on non-target aquatic organisms and potential risk to humans via contaminated foodstuffs. Additionally, this Special Issue also presents novel contaminant treatment/degradation methods of both physical and biological nature.

Cancer Metabolomics 2018

Authors: --- ---
ISBN: 9783039213450 / 9783039213467 Year: Pages: 184 DOI: 10.3390/books978-3-03921-346-7 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Biology --- Biochemistry
Added to DOAB on : 2019-12-09 11:49:15
License:

Loading...
Export citation

Choose an application

Abstract

The metabolomics approach, defined as the study of all endogenously-produced low-molecular-weight compounds, appeared as a promising strategy to define new cancer biomarkers. Information obtained from metabolomic data can help to highlight disrupted cellular pathways and, consequently, contribute to the development of new-targeted therapies and the optimization of therapeutics. Therefore, metabolomic research may be more clinically translatable than other omics approaches, since metabolites are closely related to the phenotype and the metabolome is sensitive to many factors. Metabolomics seems promising to identify key metabolic pathways characterizing features of pathological and physiological states. Thus, knowing that tumor metabolism markedly differs from the metabolism of normal cells, the use of metabolomics is ideally suited for biomarker research. Some works have already focused on the application of metabolomic approaches to different cancers, namely lung, breast and liver, using urine, exhaled breath and blood. In this Special Issue we contribute to a more complete understanding of cancer disease using metabolomics approaches.

Marine Biotoxins and Seafood Poisoning

Authors: --- ---
ISBN: 9783039218189 / 9783039218196 Year: Pages: 114 DOI: 10.3390/books978-3-03921-819-6 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Medicine (General) --- Public Health
Added to DOAB on : 2019-12-09 11:49:16
License:

Loading...
Export citation

Choose an application

Abstract

Marine biotoxins may pose a threat to the human consumption of seafood and seafood products. The increasing global trade and higher demand for seafood products worldwide represents a challenge for food safety authorities, policy makers, food business operators, and the scientific community, in particular, researchers devoted to environmental sciences, toxicology, and analytical chemistry. In addition, due to changes in climate conditions and technological developments, new and emerging marine toxins are being detected in regions where they were previously unknown. This Special Issue highlight studies aiming to the develop detection methods for marine biotoxins for better understanding the dynamics of accumulation/elimination of marine biotoxins and their effects on marine organisms, as well as toxin exposure studies that aim to evaluate the risks associated with the consumption of contaminated seafood.

Listing 1 - 10 of 21 << page
of 3
>>
Sort by
Narrow your search

Publisher

MDPI - Multidisciplinary Digital Publishing Institute (20)

Springer (1)


License

CC by-nc-nd (20)

Springer (1)


Language

eng (20)

english (1)


Year
From To Submit

2019 (19)

2018 (1)

2014 (1)