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LC-MS/MS Method for Mycotoxin Analysis

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ISBN: 9783038426066 9783038426073 Year: Pages: VIII, 186 Language: English
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Chemistry (General)
Added to DOAB on : 2017-12-22 11:28:33
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Abstract

Mycotoxins are secondary metabolites produced by some species of filamentous fungi belonging to Fusarium, Aspergillus, Penicillium, Claviceps and Alternaria genera. The biochemical significance of mycotoxins in fungal metabolism has not always been fully clarified; however, the main concern for mycotoxins is driven by the deleterious effects they can exert on human and animal health. Structurally, the mycotoxins currently known belong to heterogeneous chemical classes, therefore the toxic effects they can induce are highly diverse (e.g., cancerogenic and immunosuppressive effects). The main route of human exposure to mycotoxins is by intake of contaminated food, either directly from contaminated agricultural products, or indirectly from residues and metabolites present in foods of animal origin. Immunochemical methods are generally used for the fast screening of mycotoxin presence; however, for confirmation purposes, analytical methods based on high-performance liquid chromatography (LC) are preferred, especially when coupled with tandem mass spectrometry (MS/MS), which allows the determination of multiclass mycotoxins in a single analysis. Moreover, the technical innovations available in LC-MS/MS instrumentation are prompting its application to the monitoring of contaminants in food and feed. The aim of this paper collection, constituted by ten research articles and one review, is to provide the reader with an overview on the novelties and capabilities in LC-MS/MS-based multi-mycotoxin methods, also including the investigation of emerging and modified mycotoxins.

Promising Detoxification Strategies to Mitigate Mycotoxins in Food and Feed

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ISBN: 9783038970286 / 9783038970279 Year: Pages: 306 DOI: 10.3390/books978-3-03897-027-9 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Medicine (General) --- Public Health
Added to DOAB on : 2019-08-28 11:21:28
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This book is a printed edition of the Special Issue Promising Detoxification Strategies to Mitigate Mycotoxins in Food and Feed that was published in Toxins

Keywords

deoxynivalenol --- epimer --- polarity --- Tri101 --- molecular --- interactions --- spores of Ganoderma lucidum --- oxidative stress --- aflatoxins --- antioxidant capability --- mycotoxin --- patulin --- biodegradation --- Pichia caribbica --- proteomics --- intracellular and extracellular enzymes --- Bacillus licheniformis CK1 --- zearalenone (ZEA) --- serum hormones --- estrogen receptor (ER) --- post-weaning female piglets --- curcumin --- aflatoxin B1 --- CYP450 --- AFBO–DNA --- chicks --- aflatoxin B1 --- photodegradation product --- TQEF-MS/MS --- cell viability --- furan rings --- mycotoxin --- toxigenic Fusarium --- biological control --- Trichoderma --- modified mycotoxin --- aflatoxin B1 --- aflatoxin biodegradation preparation --- Bacillus subtilis ANSB060 --- ameliorating effects --- growth performance --- antioxidant function --- residue --- aflatoxins --- biotransformation --- enzymatic detoxification --- laccase --- mild technologies --- food safety --- mycotoxins mitigation --- aflatoxin B1 --- aflatoxin-degrading enzyme --- biodegradation --- Bacillus shackletonii --- purification --- Sporobolomyces sp. IAM 13481 --- microbial patulin degradation --- desoxypatulinic acid --- ascladiol --- aflatoxins --- Aspergillus flavus --- Corylus avellana --- fatty acids --- thermal treatment --- Aflatoxin B1 --- Aspergillus flavus --- hyssop --- inhibition --- oxidative stress --- DBD --- atmospheric pressure --- low temperature plasma --- mycotoxins --- degradation --- maize --- aflatoxins --- neutral electrolyzed water --- detoxification --- turkey --- mycotoxins --- biotransformation --- degradation --- enzymes --- application --- mycotoxin --- detoxification --- biodegradation --- biotransformation --- enzyme --- microorganism identification --- mycotoxin --- trichothecene --- deoxynivalenol --- bioprospecting --- detoxification --- Fusarium --- cold atmospheric pressure plasma technology --- mycotoxins --- physical decontamination --- chemical decontamination --- biological decontamination --- patulin --- mycotoxin --- mitigation --- decontamination --- food and beverage --- processing --- n/a

Effects of Mycotoxins on the Intestine

Authors: --- ---
ISBN: 9783038977827 9783038977834 Year: Pages: 262 DOI: 10.3390/books978-3-03897-783-4 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Medicine (General) --- Public Health
Added to DOAB on : 2019-05-09 17:16:14
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Abstract

Mycotoxins are secondary metabolites produced by several fungal species. They can contaminate human food and animal feed, and have been a threat for thousands of years. The gastrointestinal tract is the first target when ingesting mycotoxin-contaminated food or feed. As unlikely as it sounds, the investigations concerning the effects of mycotoxins on the intestine are still in their early stages. This book gathers the most recent advances related to the characterization of the intestinal toxicity of mycotoxins. Substantial data assembled on the damage caused to a number of histological structures and functions of the intestine remove any remaining doubt about this organ being a primary target for the toxicity of mycotoxins. An interesting overview of the detrimental effects of mycotoxins on the gut-hosted microbiota—now regarded as a fully-fledged organ associated with the gut—is also given. Finally, outstanding contributions in this book address questions relating to the suitability of current regulations to protect against alterations of the intestine, and to the efficacy assessment of new detoxification strategies using the intestinal toxicity of mycotoxins as a relevant endpoint.

Keywords

mice --- aflatoxin B1 --- intestinal bacterial flora --- response --- Clostridium sp. WJ06 --- deoxynivalenol --- pig --- intestinal morphology --- microbial diversity --- aflatoxin M1 --- ochratoxin A --- intestinal epithelial cells --- tight junction --- permeability --- ileum --- jejunum --- deoxynivalenol --- piglet --- contaminated feed --- tight junction --- aflatoxin B1 --- small intestine --- histopathological lesions --- ultrastructural changes --- toll-like receptors --- T-2 toxin --- enteric nervous system --- pig --- vasoactive intestinal polypeptide --- mycotoxins --- zearalenone --- deoxynivalenol --- histology --- ultrastructure --- large intestine --- pig --- Claviceps --- liver --- digestive tract --- mycotoxin --- sclerotia --- ergot alkaloids --- toxicity --- deoxynivalenol --- Saccharomyces cerevisiae boulardii CNCM I-1079 --- intestine --- transcriptome --- inflammation --- oxidative stress --- lipid metabolism --- fumonisin --- microbiota --- pigs --- MiSeq 16S rDNA sequencing --- intestinal microbiota --- hydrogen-rich water --- lactulose --- Fusarium mycotoxins --- piglets --- functional oligosaccharides --- mycotoxins --- swine --- explant technique --- intestinal morphology --- goblet cells --- deoxynivalenol --- zearalenone --- pig --- colon microbiota --- Lactobacillus --- detoxification --- zearalenone --- doses --- caecal water --- genotoxicity --- pre-pubertal gilts --- atlantic salmon --- deoxynivalenol --- feed --- intestine --- PCR --- proliferating cell nuclear antigen --- suppressor of cytokine signaling --- tight junctions --- Zearalenone --- N-acetylcysteine --- SIEC02 cells --- Mitochondrial apoptosis --- n/a

Advances in Food Analysis

Authors: ---
ISBN: 9783039217427 / 9783039217434 Year: Pages: 488 DOI: 10.3390/books978-3-03921-743-4 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Mathematics
Added to DOAB on : 2019-12-09 11:49:16
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Abstract

This Topical Collection of Molecules provides the most recent advancements and trends within the framework of food analysis, confirming the growing public, academic, and industrial interest in this field. The articles broach topics related to sample preparation, separation science, spectroscopic techniques, sensors and biosensors, as well as investigations dealing with the characterization of macronutrients, micronutrients, and other biomolecules. It offers the latest updates regarding alternative food sources (e.g., algae), functional foods, effects of processing, chiral or achiral bioactive compounds, contaminants, and every topic related to food science that is appealing to readers. Nowadays, the increasing awareness of the close relation among diet, health, and social development is stimulating demands for high levels of quality and safety in agro-food production, as well as new studies to fill gaps in the actual body of knowledge about food composition. For these reasons, modern research in food science and human nutrition is moving from classical methodologies to advanced instrumental platforms for comprehensive characterization. Nondestructive spectroscopic and imaging technologies are also proposed for food process monitoring and quality control in real time.

Keywords

cuprous oxide nanoparticles --- reduced graphene oxide --- modified electrode --- sunset yellow --- second-derivative linear sweep voltammetry --- clenbuterol --- systematic evolution of ligands by exponential enrichment --- real-time quantitative PCR --- high-throughput sequencing technology --- aptamers --- gold nanoparticles biosensor --- carbamates --- multiple reaction monitoring (MRM) --- enhanced product ion (EPI) --- mass fragmentation --- confirmatory method --- pesticide residues --- Croatian wines --- biogenic amines --- HPLC --- geographical origin --- polyelectrolyte composite film --- nitrite detection --- differential pulse voltammetry --- cyclic voltammetry --- mycotoxin --- dimerization --- HRMS --- NMR --- fruit jams --- food security --- phenolic acids --- quercetin --- agro-biodiversity --- HPLC fingerprint --- Polygonatum cyrtonema --- saccharides --- oligosaccharides --- fructose --- HPLC–QTOF–MS/MS --- steaming --- essential oil --- extraction techniques --- hops extracts --- hydrodistillation --- Marynka strain --- microwave-assisted hydrodistillation --- anthocyanins --- bioactive compounds --- Box–Behnken design --- microwave-assisted extraction --- myrtle --- Myrtus communis --- phenolic compounds --- Chia seed oil --- polyunsaturated fatty acid --- antioxidant --- lipid-lowering effect --- collagen peptide --- HPLC fingerprint --- antioxidant --- anti-inflammatory --- spectrum-effect relationship --- amino acids --- carbohydrates --- acidity --- polarity --- molecular weight --- Tricholoma matsutake --- Pol gene --- qualitative and quantitative PCR --- DNA extraction --- ?-blockers --- metabolites --- milk powder --- Q-Orbitrap --- rosé wines --- white wines --- bottle aging --- flavor profile --- closures --- anthocyanins --- bioactive compounds --- Box–Behnken design --- ultrasound-assisted extraction --- myrtle --- Myrtus communis L. --- phenolic compounds --- food safety --- kiwifruit (Actinidia chinensis) --- molecular identification --- phylogeny --- DNA barcode --- hard clams --- Meretrix lyrata --- lipid classes --- fatty acids --- phospholipids --- molecular species of phospholipid --- high resolution mass spectrometry --- impedimetric aptasensor --- screen-printed interface --- bifunctional polymer arms --- PAT detection --- apple juice --- chiral --- chiral stationary phases --- enantiomers --- food --- review --- Piper methysticum (kava) --- kavalactones --- flavokavains --- UHPLC-UV --- mass spectra --- isomerization --- single-laboratory validation --- quality control --- Lactarius deliciosus --- chemical composition --- antioxidant --- antihyperglycemic --- ?13C-IRMS --- fatty acids composition --- 1H-NMR --- walnut varieties --- poultry eggs --- thiamphenicol --- florfenicol --- florfenicol amine --- ASE --- UPLC-FLD --- Sojae semen praeparatum (SSP) --- fermentation --- conversion --- ultra-fast liquid chromatography (UFLC)–TripleTOF MS --- principal component analysis (PCA) --- microalgae --- Scenedesmus --- supercritical fluid extraction --- carotenoids --- fat-soluble vitamins --- antioxidants --- fruit juice --- blends --- adulteration --- 1H NMR --- PLS --- chemometrics --- natural mature honey --- immature honey --- chemometric analysis --- multi-physicochemical parameters --- food quality --- IMS --- food composition --- food process control --- food authentication --- food adulteration --- food safety --- antibiotics --- liquid chromatography mass spectrometry --- milk --- muscle --- validation

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